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Sample GSM115051 Query DataSets for GSM115051
Status Public on Jul 10, 2006
Title Monocyte at 3 days, biological rep3 HG-U133A
Sample type RNA
 
Source name Blood
Organism Homo sapiens
Characteristics Monocyte at 3 days, biological rep3
Treatment protocol monocytes stimulated for 3 days with 100 ng/ml M-CSF
Growth protocol RPMI 1640 20%FCS 100 ng/ml M-CSF
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
 
Submission date Jun 19, 2006
Last update date Jul 15, 2019
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL96
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
Relations
Reanalyzed by GSE86363
Reanalyzed by GSE134312

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
1007_s_at 7.767454159
1053_at 8.360598339
117_at 9.137594459
121_at 9.763873811
1255_g_at 4.71280492
1294_at 9.161645873
1316_at 6.671499432
1320_at 5.346673806
1405_i_at 5.596201126
1431_at 4.256534292
1438_at 7.166405131
1487_at 9.130921495
1494_f_at 6.974727752
1598_g_at 9.909732393
160020_at 9.354141276
1729_at 9.408620104
177_at 6.96223213
1773_at 6.765070297
179_at 9.27423452
1861_at 7.034676202

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM115051_Md3_D3_chipA.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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