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Sample GSM115054 Query DataSets for GSM115054
Status Public on Jul 10, 2006
Title Macrophage at 7 days, biological rep3 HG-U133A
Sample type RNA
 
Source name Blood
Organism Homo sapiens
Characteristics Macrophage at 7 days, biological rep3
Treatment protocol monocytes stimulated for 7 days with 100 ng/ml M-CSF
Growth protocol RPMI 1640 20%FCS 100 ng/ml M-CSF
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
 
Submission date Jun 19, 2006
Last update date Jul 15, 2019
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL96
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
Relations
Reanalyzed by GSE86363
Reanalyzed by GSE134312

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
1007_s_at 8.317145487
1053_at 7.970467047
117_at 8.833635624
121_at 9.849997175
1255_g_at 4.728047694
1294_at 9.0948828
1316_at 7.088191223
1320_at 5.579743248
1405_i_at 6.212434213
1431_at 4.387124682
1438_at 7.452529644
1487_at 8.711365783
1494_f_at 7.24223788
1598_g_at 11.42931215
160020_at 9.856891216
1729_at 9.218359395
177_at 7.075751824
1773_at 7.54349761
179_at 9.858450766
1861_at 7.027776747

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM115054_Mph_D3_chipA.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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