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Sample GSM115057 Query DataSets for GSM115057
Status Public on Jul 10, 2006
Title classical or M1 activated macrophages, biological rep3 HG-U133A
Sample type RNA
Source name Blood
Organism Homo sapiens
Characteristics classical or M1 activated macrophages, biological rep3
Treatment protocol mature macrophages supplemented with 20 ng/ml IFN-g+ 100 ng/ml LPS
Growth protocol RPMI 1640 20%FCS 100 ng/ml M-CSF
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
Submission date Jun 19, 2006
Last update date Jul 15, 2019
Contact name Fernando O Martinez Estrada
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
Platform ID GPL96
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
Reanalyzed by GSE86363
Reanalyzed by GSE134312

Data table header descriptions
VALUE RMA-calculated Signal intensity

Data table
1007_s_at 9.862679252
1053_at 8.267905892
117_at 8.097417106
121_at 9.590382072
1255_g_at 4.871845178
1294_at 9.261369769
1316_at 7.0064187
1320_at 5.731490621
1405_i_at 12.49465567
1431_at 4.449145633
1438_at 7.384578414
1487_at 8.87696854
1494_f_at 7.537720138
1598_g_at 11.32453321
160020_at 9.94045611
1729_at 11.27025671
177_at 7.271893909
1773_at 7.007552426
179_at 9.854446543
1861_at 6.622758356

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.

Supplementary file Size Download File type/resource
GSM115057_M1_D3_chipA.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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