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Sample GSM115058 Query DataSets for GSM115058
Status Public on Jul 10, 2006
Title Alternative or M2 activated macrophages, biological rep1 HG-U133A
Sample type RNA
 
Source name Blood
Organism Homo sapiens
Characteristics ALternative or M2 activated macrophages, biological rep1
Treatment protocol mature macrophages supplemented with 20 ng/ml IL-4
Growth protocol mature macrophages supplemented with 20 ng/ml IFN-g+ 100 ng/ml LPS
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
 
Submission date Jun 19, 2006
Last update date Jul 15, 2019
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL96
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated
Relations
Reanalyzed by GSE86363
Reanalyzed by GSE134312

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
1007_s_at 8.370963649
1053_at 8.167642206
117_at 8.374230368
121_at 9.664449971
1255_g_at 4.622558206
1294_at 8.818580053
1316_at 6.945585347
1320_at 5.696356383
1405_i_at 6.518416039
1431_at 4.353758669
1438_at 7.424451863
1487_at 8.940185297
1494_f_at 7.400508451
1598_g_at 12.86234209
160020_at 10.42477561
1729_at 9.24376884
177_at 6.935865742
1773_at 7.710000876
179_at 9.929351464
1861_at 7.04663258

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM115058_M2_D1_chipA.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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