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Sample GSM115061 Query DataSets for GSM115061
Status Public on Jul 10, 2006
Title Monocyte at T0, biological rep1 HG-U133B
Sample type RNA
 
Source name Blood
Organism Homo sapiens
Characteristics Monocyte at T0, biological rep1
Treatment protocol freshly isolated monocytes
Growth protocol Cells were cultured in Petriperm dishes tomimic circulating conditions
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
 
Submission date Jun 19, 2006
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL97
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
200000_s_at 9.391244692
200001_at 11.06285478
200002_at 11.13280733
200003_s_at 12.24769923
200004_at 11.36172822
200005_at 10.56246391
200006_at 10.29885472
200007_at 10.22467496
200008_s_at 10.20996533
200009_at 11.43138858
200010_at 11.26389595
200011_s_at 9.954851591
200012_x_at 11.66274638
200013_at 11.2109426
200014_s_at 9.6826417
200015_s_at 9.4890872
200016_x_at 11.37989845
200017_at 11.11906719
200018_at 12.12727785
200019_s_at 11.72376573

Total number of rows: 22645

Table truncated, full table size 492 Kbytes.




Supplementary file Size Download File type/resource
GSM115061_M0_D1_chipB.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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