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Sample GSM115064 Query DataSets for GSM115064
Status Public on Jul 10, 2006
Title Monocyte at 3 days, biological rep1 HG-U133B
Sample type RNA
 
Source name Blood
Organism Homo sapiens
Characteristics Monocyte at 3 days, biological rep1
Treatment protocol monocytes stimulated for 3 days with 100 ng/ml M-CSF
Growth protocol RPMI 1640 20%FCS 100 ng/ml M-CSF
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
 
Submission date Jun 19, 2006
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL97
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
200000_s_at 9.321871142
200001_at 11.27622069
200002_at 10.91826596
200003_s_at 11.71668876
200004_at 10.66633861
200005_at 10.41589011
200006_at 10.405065
200007_at 10.63177534
200008_s_at 9.97071143
200009_at 11.32590034
200010_at 10.91816118
200011_s_at 10.4648882
200012_x_at 11.19747996
200013_at 10.77971956
200014_s_at 9.716191036
200015_s_at 9.417518276
200016_x_at 10.89013954
200017_at 10.67540986
200018_at 11.84314013
200019_s_at 11.00615844

Total number of rows: 22645

Table truncated, full table size 492 Kbytes.




Supplementary file Size Download File type/resource
GSM115064_Md3_D1_chipB.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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