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Sample GSM115065 Query DataSets for GSM115065
Status Public on Jul 10, 2006
Title Monocyteat 3 days, biological rep2 HG-U133B
Sample type RNA
 
Source name Blood
Organism Homo sapiens
Characteristics Monocyteat 3 days, biological rep2
Treatment protocol monocytes stimulated for 3 days with 100 ng/ml M-CSF
Growth protocol RPMI 1640 20%FCS 100 ng/ml M-CSF
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
 
Submission date Jun 19, 2006
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL97
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
200000_s_at 9.606258651
200001_at 11.68550211
200002_at 11.24104399
200003_s_at 11.79082621
200004_at 11.24884293
200005_at 10.61406386
200006_at 10.79172007
200007_at 10.90600337
200008_s_at 10.35096921
200009_at 11.6147844
200010_at 11.3160374
200011_s_at 10.64739453
200012_x_at 11.51622833
200013_at 11.16872768
200014_s_at 10.31988527
200015_s_at 9.952323931
200016_x_at 11.28699572
200017_at 11.04951915
200018_at 12.15674625
200019_s_at 11.31860068

Total number of rows: 22645

Table truncated, full table size 492 Kbytes.




Supplementary file Size Download File type/resource
GSM115065_Md3_D2_chipB.CEL.gz 3.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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