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Sample GSM115068 Query DataSets for GSM115068
Status Public on Jul 10, 2006
Title Macrophage at 7 days, biological rep2 HG-U133B
Sample type RNA
 
Source name Blood
Organism Homo sapiens
Characteristics Macrophage at 7 days, biological rep2
Treatment protocol monocytes stimulated for 7 days with 100 ng/ml M-CSF
Growth protocol RPMI 1640 20%FCS 100 ng/ml M-CSF
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
 
Submission date Jun 19, 2006
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL97
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
200000_s_at 9.538957263
200001_at 10.92919972
200002_at 11.24095552
200003_s_at 11.9919549
200004_at 11.01339571
200005_at 10.54207923
200006_at 10.23519307
200007_at 10.63146506
200008_s_at 9.946589774
200009_at 11.14401298
200010_at 11.47011792
200011_s_at 10.16972843
200012_x_at 11.9262433
200013_at 11.19392124
200014_s_at 9.858997125
200015_s_at 9.783390516
200016_x_at 11.2838012
200017_at 11.39702499
200018_at 12.05940234
200019_s_at 11.24240816

Total number of rows: 22645

Table truncated, full table size 492 Kbytes.




Supplementary file Size Download File type/resource
GSM115068_Mph_D2_chipB.CEL.gz 3.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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