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Sample GSM115071 Query DataSets for GSM115071
Status Public on Jul 10, 2006
Title classical or M1 activated macrophages, biological rep2 HG-U133B
Sample type RNA
 
Source name Blood
Organism Homo sapiens
Characteristics classical or M1 activated macrophages, biological rep2
Treatment protocol mature macrophages supplemented with 20 ng/ml IFN-g+ 100 ng/ml LPS
Growth protocol mature macrophages supplemented with 20 ng/ml IFN-g+ 100 ng/ml LPS
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
 
Submission date Jun 19, 2006
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL97
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
200000_s_at 8.224032429
200001_at 10.95569709
200002_at 10.18452249
200003_s_at 11.78510397
200004_at 10.70997294
200005_at 9.704247943
200006_at 9.825839926
200007_at 10.57606155
200008_s_at 9.569137929
200009_at 10.74063539
200010_at 10.53847874
200011_s_at 10.52073818
200012_x_at 10.85413337
200013_at 10.38644345
200014_s_at 9.224949641
200015_s_at 8.796955538
200016_x_at 10.57791281
200017_at 10.77575737
200018_at 11.06054848
200019_s_at 10.33967367

Total number of rows: 22645

Table truncated, full table size 492 Kbytes.




Supplementary file Size Download File type/resource
GSM115071_M1_D2_chipB.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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