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Sample GSM115072 Query DataSets for GSM115072
Status Public on Jul 10, 2006
Title classical or M1 activated macrophages, biological rep3 HG-U133B
Sample type RNA
 
Source name Blood
Organism Homo sapiens
Characteristics classical or M1 activated macrophages, biological rep3
Treatment protocol mature macrophages supplemented with 20 ng/ml IFN-g+ 100 ng/ml LPS
Growth protocol RPMI 1640 20%FCS 100 ng/ml M-CSF
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
 
Submission date Jun 19, 2006
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL97
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
200000_s_at 8.397772957
200001_at 11.04484138
200002_at 10.70734107
200003_s_at 12.09165364
200004_at 10.84601732
200005_at 10.00290055
200006_at 10.0920596
200007_at 10.59836775
200008_s_at 9.301133537
200009_at 11.04113296
200010_at 10.50650338
200011_s_at 10.75073101
200012_x_at 11.21976742
200013_at 10.41290873
200014_s_at 9.594026174
200015_s_at 9.230207775
200016_x_at 10.85650953
200017_at 10.81351334
200018_at 11.29130119
200019_s_at 10.58637831

Total number of rows: 22645

Table truncated, full table size 492 Kbytes.




Supplementary file Size Download File type/resource
GSM115072_M1_D3_chipB.CEL.gz 3.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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