|
| Status |
Public on Jul 10, 2006 |
| Title |
classical or M1 activated macrophages, biological rep3 HG-U133B |
| Sample type |
RNA |
| |
|
| Source name |
Blood
|
| Organism |
Homo sapiens |
| Characteristics |
classical or M1 activated macrophages, biological rep3
|
| Treatment protocol |
mature macrophages supplemented with 20 ng/ml IFN-g+ 100 ng/ml LPS
|
| Growth protocol |
RPMI 1640 20%FCS 100 ng/ml M-CSF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Monocytes
|
| Data processing |
The data were analyzed with RNA using Bioconductor's R.
|
| |
|
| Submission date |
Jun 19, 2006 |
| Last update date |
Apr 12, 2018 |
| Contact name |
Fernando O Martinez Estrada |
| E-mail(s) |
f.martinezestrada@surrey.ac.uk
|
| Organization name |
University of Surrey
|
| Department |
Faculty of Health & Medical Sciences
|
| Lab |
Innate Immunity
|
| Street address |
Stag Hill
|
| City |
Guildford |
| State/province |
Surrey |
| ZIP/Postal code |
GU2 7XH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL97 |
| Series (2) |
| GSE5099 |
Expression Data from Macrophage Maturation and Polarization Experiment |
| GSE35495 |
Genome-wide analysis of human and mouse macrophages, resting and activated |
|
