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Sample GSM115074 Query DataSets for GSM115074
Status Public on Jul 10, 2006
Title Alternative or M2 activated macrophages, biological rep2 HG-U133B
Sample type RNA
 
Source name Blood
Organism Homo sapiens
Characteristics ALternative or M2 activated macrophages, biological rep2
Treatment protocol mature macrophages supplemented with 20 ng/ml IL-4
Growth protocol mature macrophages supplemented with 20 ng/ml IFN-g+ 100 ng/ml LPS
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HGU133 series. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Monocytes
Data processing The data were analyzed with RNA using Bioconductor's R.
 
Submission date Jun 19, 2006
Last update date Apr 12, 2018
Contact name Fernando O Martinez Estrada
E-mail(s) f.martinezestrada@surrey.ac.uk
Organization name University of Surrey
Department Faculty of Health & Medical Sciences
Lab Innate Immunity
Street address Stag Hill
City Guildford
State/province Surrey
ZIP/Postal code GU2 7XH
Country United Kingdom
 
Platform ID GPL97
Series (2)
GSE5099 Expression Data from Macrophage Maturation and Polarization Experiment
GSE35495 Genome-wide analysis of human and mouse macrophages, resting and activated

Data table header descriptions
ID_REF
VALUE RMA-calculated Signal intensity

Data table
ID_REF VALUE
200000_s_at 9.289744705
200001_at 11.06856593
200002_at 11.06636567
200003_s_at 12.11282078
200004_at 10.92093846
200005_at 10.95336006
200006_at 10.06912462
200007_at 10.71048471
200008_s_at 9.61944982
200009_at 11.14401298
200010_at 11.37027669
200011_s_at 10.26501477
200012_x_at 11.66274638
200013_at 11.02896824
200014_s_at 9.654908453
200015_s_at 9.298330883
200016_x_at 11.00667899
200017_at 11.27961033
200018_at 11.98683698
200019_s_at 11.11061017

Total number of rows: 22645

Table truncated, full table size 492 Kbytes.




Supplementary file Size Download File type/resource
GSM115074_M2_D2_chipB.CEL.gz 3.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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