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Sample GSM1151931 Query DataSets for GSM1151931
Status Public on Dec 31, 2013
Title IL13_matrigel_rep1
Sample type RNA
 
Channel 1
Source name IMR90, matrigel, IL13
Organism Homo sapiens
Characteristics cell line: IMR90
cell type: lung fibroblasts
substrate: matrigel
treatment: IL13
timepoint: 24h
Treatment protocol Cells were treated for 24 hours with IL-4 (10 ng/ml), IL-13 (10ng/ml), or TNFa (10 ng/ml).
Growth protocol IMR-90 cells (ATCC, Manassas, VA; Catalog # CCL-186) were cultured in DMEM medium supplemented with 10% FBS (Sigma, St. Louis, MO; Catalog # F2442) and Penicillin/Streptomycin (Invitrogen, Carlsbad, CA; Catalog # 15140) on a bed of growth factor reduced Matrigel (GFR Matrigel, BD Biosciences, Bedford, MA; Catalog No. 354230) or plastic.
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Qiagen).
Label Cy5
Label protocol Biotinylated cRNA were prepared according to the standard Agilent protocol.
 
Channel 2
Source name Stratagene Universal Human Reference RNA
Organism Homo sapiens
Characteristics sample type: reference
Extracted molecule total RNA
Extraction protocol Stratagene Universal Human Reference RNA (catalog number 740000).
Label Cy3
Label protocol Biotinylated cRNA were prepared according to the standard Agilent protocol.
 
 
Hybridization protocol cRNA was hybridized on Agilent-014850 Whole Human Genome Microarray G4112F.
Scan protocol Chips were scanned using the Agilent Surescan Microarray Scanner.
Description SAM630152
treated with IL13 on matrigel
Data processing The data were processed with Agilent Feature Extraction software, version 10.7.3.1, using default settings, including linear and Lowess normalization.
 
Submission date May 30, 2013
Last update date Dec 31, 2013
Contact name Alex Abbas
E-mail(s) abbas@gene.com
Organization name Genentech, Inc.
Department Bioinformatics
Street address 1 DNA Way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
 
Platform ID GPL6480
Series (1)
GSE47542 IMR90 lung fibroblasts treated with IL-4 or IL-13

Data table header descriptions
ID_REF
VALUE Agilent log2 ratio, test channel (ch1)/reference channel (ch2).

Data table
ID_REF VALUE
A_23_P100001 -3.7727
A_23_P100011 -0.24487
A_23_P100022 -0.76752
A_23_P100056 -1.6219
A_23_P100074 1.3789
A_23_P100092 0.28634
A_23_P100103 1.1009
A_23_P100111 1.3545
A_23_P100127 -3.3644
A_23_P100133 0.54906
A_23_P100141 0.8476
A_23_P100156 0.99556
A_23_P100177 -0.32695
A_23_P100189 -0.099894
A_23_P100196 -1.7674
A_23_P100203 0.96746
A_23_P100220 -3.365
A_23_P100240 -3.5351
A_23_P10025 -4.6987
A_23_P100263 2.0755

Total number of rows: 41107

Table truncated, full table size 807 Kbytes.




Supplementary file Size Download File type/resource
GSM1151931_Agilent_251485054012_S01_GE2_107_Sep09_1_1.txt.gz 4.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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