NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1151939 Query DataSets for GSM1151939
Status Public on Dec 31, 2013
Title unstim_plastic_rep1
Sample type RNA
 
Channel 1
Source name IMR90, plastic, unstimulated
Organism Homo sapiens
Characteristics cell line: IMR90
cell type: lung fibroblasts
substrate: plastic
treatment: unstimulated
timepoint: 24h
Treatment protocol Cells were treated for 24 hours with IL-4 (10 ng/ml), IL-13 (10ng/ml), or TNFa (10 ng/ml).
Growth protocol IMR-90 cells (ATCC, Manassas, VA; Catalog # CCL-186) were cultured in DMEM medium supplemented with 10% FBS (Sigma, St. Louis, MO; Catalog # F2442) and Penicillin/Streptomycin (Invitrogen, Carlsbad, CA; Catalog # 15140) on a bed of growth factor reduced Matrigel (GFR Matrigel, BD Biosciences, Bedford, MA; Catalog No. 354230) or plastic.
Extracted molecule total RNA
Extraction protocol RNeasy Mini Kit (Qiagen).
Label Cy5
Label protocol Biotinylated cRNA were prepared according to the standard Agilent protocol.
 
Channel 2
Source name Stratagene Universal Human Reference RNA
Organism Homo sapiens
Characteristics sample type: reference
Extracted molecule total RNA
Extraction protocol Stratagene Universal Human Reference RNA (catalog number 740000).
Label Cy3
Label protocol Biotinylated cRNA were prepared according to the standard Agilent protocol.
 
 
Hybridization protocol cRNA was hybridized on Agilent-014850 Whole Human Genome Microarray G4112F.
Scan protocol Chips were scanned using the Agilent Surescan Microarray Scanner.
Description SAM630160
treated with unstim on plastic
Data processing The data were processed with Agilent Feature Extraction software, version 10.7.3.1, using default settings, including linear and Lowess normalization.
 
Submission date May 30, 2013
Last update date Dec 31, 2013
Contact name Alex Abbas
E-mail(s) abbas@gene.com
Organization name Genentech, Inc.
Department Bioinformatics
Street address 1 DNA Way
City South San Francisco
State/province CA
ZIP/Postal code 94080
Country USA
 
Platform ID GPL6480
Series (1)
GSE47542 IMR90 lung fibroblasts treated with IL-4 or IL-13

Data table header descriptions
ID_REF
VALUE Agilent log2 ratio, test channel (ch1)/reference channel (ch2).

Data table
ID_REF VALUE
A_23_P100001 -3.4309
A_23_P100011 0.12153
A_23_P100022 -0.91683
A_23_P100056 -1.5164
A_23_P100074 1.5896
A_23_P100092 0.45424
A_23_P100103 0.99945
A_23_P100111 0.93916
A_23_P100127 0.54798
A_23_P100133 0.28574
A_23_P100141 0.43685
A_23_P100156 0.56337
A_23_P100177 -1.0486
A_23_P100189 0.16659
A_23_P100196 -0.57431
A_23_P100203 0.11275
A_23_P100220 -3.1114
A_23_P100240 -3.3864
A_23_P10025 -3.2345
A_23_P100263 0.48358

Total number of rows: 41107

Table truncated, full table size 814 Kbytes.




Supplementary file Size Download File type/resource
GSM1151939_Agilent_251485053931_S01_GE2_107_Sep09_1_3.txt.gz 4.4 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap