|
| Status |
Public on Dec 31, 2013 |
| Title |
unstim_plastic_rep3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
IMR90, plastic, unstimulated
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: IMR90
cell type: lung fibroblasts
substrate: plastic
treatment: unstimulated
timepoint: 24h
|
| Treatment protocol |
Cells were treated for 24 hours with IL-4 (10 ng/ml), IL-13 (10ng/ml), or TNFa (10 ng/ml).
|
| Growth protocol |
IMR-90 cells (ATCC, Manassas, VA; Catalog # CCL-186) were cultured in DMEM medium supplemented with 10% FBS (Sigma, St. Louis, MO; Catalog # F2442) and Penicillin/Streptomycin (Invitrogen, Carlsbad, CA; Catalog # 15140) on a bed of growth factor reduced Matrigel (GFR Matrigel, BD Biosciences, Bedford, MA; Catalog No. 354230) or plastic.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen).
|
| Label |
Cy5
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Agilent protocol.
|
| |
|
| Channel 2 |
| Source name |
Stratagene Universal Human Reference RNA
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: reference
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Stratagene Universal Human Reference RNA (catalog number 740000).
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Agilent protocol.
|
| |
|
| |
| Hybridization protocol |
cRNA was hybridized on Agilent-014850 Whole Human Genome Microarray G4112F.
|
| Scan protocol |
Chips were scanned using the Agilent Surescan Microarray Scanner.
|
| Description |
SAM630168
treated with unstim on plastic
|
| Data processing |
The data were processed with Agilent Feature Extraction software, version 10.7.3.1, using default settings, including linear and Lowess normalization.
|
| |
|
| Submission date |
May 30, 2013 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Alex Abbas |
| E-mail(s) |
abbas@gene.com
|
| Organization name |
Genentech, Inc.
|
| Department |
Bioinformatics
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE47542 |
IMR90 lung fibroblasts treated with IL-4 or IL-13 |
|
