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Sample GSM1152577 Query DataSets for GSM1152577
Status Public on Jun 01, 2014
Title 0.01µg/ml 1 month rep 2
Sample type RNA
Source name mouse testis following 0.01 µg/ml acrylamide for 1 month
Organism Mus musculus
Characteristics strain: Swiss
gender: Male
age: 3-6 months
agent: acrylamide
dose: 0.01 µg/ml
time: 1 month
tissue: testis
Treatment protocol Acrylamide drinking water solutions were prepared using research-grade acrylamide obtained from Sigma (≥ 99% purity, A9099) diluted in filtered, deionized water at concentrations 0.001, 0.01, 0.1, 1, and 10 ?g/ml. Drinking water containing acrylamide was prepared fresh on a weekly basis, and was substituted for normal drinking water at the commencement of treatment. Test animals were exposed to acrylamide via the drinking water for a period of 1, 6 or 12 months
Growth protocol Adult male Swiss mice were housed under controlled temperature and humidity conditions, 16 h light, 8 h dark, with food and water provided ad libitum. Animals were acclimated for at least 1 month prior to treatment. At each time point, animals were euthanized by CO2 asphyxiation and testes were extracted.
Extracted molecule total RNA
Extraction protocol RNA was extracted from individual testes of acrylamide exposed mice using RNeasy Mini Kit (Qiagen, Chadstone Centre, VIC) according to manufacturers' instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared with Ambion's Illumina RNA Amplification kit for Illumina arrays
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Sample name: 8
Data processing The data were normalised using quantile normalisation using BioConductor's lumi package in R statistical software
Submission date May 31, 2013
Last update date Jun 01, 2014
Contact name Belinda Nixon
Organization name University of Newcastle
Street address University Drive
City Callaghan
State/province NSW
ZIP/Postal code 2308
Country Australia
Platform ID GPL6885
Series (1)
GSE47574 Expression data from mouse testis following chronic exposure to acrylamide via the drinking water

Data table header descriptions
VALUE Quantile normalized signal intensity

Data table
ILMN_1250052 10.01853673
ILMN_3122480 8.453643118
ILMN_2599935 8.176946977
ILMN_2675543 7.732409319
ILMN_2686883 7.735709947
ILMN_2751818 7.713583711
ILMN_2728634 7.752306888
ILMN_3040515 7.747588621
ILMN_2711608 7.701944422
ILMN_1232875 7.708362553
ILMN_1258507 7.975862865
ILMN_2746142 7.710444639
ILMN_1252690 7.963829545
ILMN_2655499 7.740552271
ILMN_1252870 7.740552271
ILMN_1248179 7.640075412
ILMN_2649955 9.14577979
ILMN_2628708 8.496826444
ILMN_3024781 7.655390298
ILMN_2705628 7.718550068

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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