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Sample GSM1152578 Query DataSets for GSM1152578
Status Public on Jun 01, 2014
Title 0.01µg/ml 1 month rep 3
Sample type RNA
 
Source name mouse testis following 0.01 µg/ml acrylamide for 1 month
Organism Mus musculus
Characteristics strain: Swiss
gender: Male
age: 3-6 months
agent: acrylamide
dose: 0.01 µg/ml
time: 1 month
tissue: testis
Treatment protocol Acrylamide drinking water solutions were prepared using research-grade acrylamide obtained from Sigma (≥ 99% purity, A9099) diluted in filtered, deionized water at concentrations 0.001, 0.01, 0.1, 1, and 10 ?g/ml. Drinking water containing acrylamide was prepared fresh on a weekly basis, and was substituted for normal drinking water at the commencement of treatment. Test animals were exposed to acrylamide via the drinking water for a period of 1, 6 or 12 months
Growth protocol Adult male Swiss mice were housed under controlled temperature and humidity conditions, 16 h light, 8 h dark, with food and water provided ad libitum. Animals were acclimated for at least 1 month prior to treatment. At each time point, animals were euthanized by CO2 asphyxiation and testes were extracted.
Extracted molecule total RNA
Extraction protocol RNA was extracted from individual testes of acrylamide exposed mice using RNeasy Mini Kit (Qiagen, Chadstone Centre, VIC) according to manufacturers' instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared with Ambion's Illumina RNA Amplification kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Sample name: 9
Data processing The data were normalised using quantile normalisation using BioConductor's lumi package in R statistical software
 
Submission date May 31, 2013
Last update date Jun 01, 2014
Contact name Belinda Nixon
E-mail(s) belinda.nixon@uon.edu.au
Organization name University of Newcastle
Street address University Drive
City Callaghan
State/province NSW
ZIP/Postal code 2308
Country Australia
 
Platform ID GPL6885
Series (1)
GSE47574 Expression data from mouse testis following chronic exposure to acrylamide via the drinking water

Data table header descriptions
ID_REF
VALUE Quantile normalized signal intensity

Data table
ID_REF VALUE
ILMN_1250052 10.26658971
ILMN_3122480 8.638025292
ILMN_2599935 8.266109397
ILMN_2675543 7.779879648
ILMN_2686883 7.756492407
ILMN_2751818 7.776444016
ILMN_2728634 7.81390765
ILMN_3040515 7.723028529
ILMN_2711608 7.717209956
ILMN_1232875 7.642123928
ILMN_1258507 7.873631637
ILMN_2746142 7.702919582
ILMN_1252690 8.053654521
ILMN_2655499 7.702919582
ILMN_1252870 7.689029349
ILMN_1248179 7.654984433
ILMN_2649955 9.035758043
ILMN_2628708 8.611992428
ILMN_3024781 7.749934674
ILMN_2705628 7.734484777

Total number of rows: 25697

Table truncated, full table size 625 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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