|
| Status |
Public on Jun 01, 2014 |
| Title |
10µg/ml 1 month rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
mouse testis following 10 µg/ml acrylamide for 1 month
|
| Organism |
Mus musculus |
| Characteristics |
strain: Swiss
gender: Male
age: 3-6 months
agent: acrylamide
dose: 10 µg/ml
time: 1 month
tissue: testis
|
| Treatment protocol |
Acrylamide drinking water solutions were prepared using research-grade acrylamide obtained from Sigma (≥ 99% purity, A9099) diluted in filtered, deionized water at concentrations 0.001, 0.01, 0.1, 1, and 10 ?g/ml. Drinking water containing acrylamide was prepared fresh on a weekly basis, and was substituted for normal drinking water at the commencement of treatment. Test animals were exposed to acrylamide via the drinking water for a period of 1, 6 or 12 months
|
| Growth protocol |
Adult male Swiss mice were housed under controlled temperature and humidity conditions, 16 h light, 8 h dark, with food and water provided ad libitum. Animals were acclimated for at least 1 month prior to treatment. At each time point, animals were euthanized by CO2 asphyxiation and testes were extracted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from individual testes of acrylamide exposed mice using RNeasy Mini Kit (Qiagen, Chadstone Centre, VIC) according to manufacturers' instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with Ambion's Illumina RNA Amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Sample name: 17
|
| Data processing |
The data were normalised using quantile normalisation using BioConductor's lumi package in R statistical software
|
| |
|
| Submission date |
May 31, 2013 |
| Last update date |
Jun 01, 2014 |
| Contact name |
Belinda Nixon |
| E-mail(s) |
belinda.nixon@uon.edu.au
|
| Organization name |
University of Newcastle
|
| Street address |
University Drive
|
| City |
Callaghan |
| State/province |
NSW |
| ZIP/Postal code |
2308 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE47574 |
Expression data from mouse testis following chronic exposure to acrylamide via the drinking water |
|
