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Sample GSM1152592 Query DataSets for GSM1152592
Status Public on Jun 01, 2014
Title 0.001µg/ml 6 month rep 3
Sample type RNA
 
Source name mouse testis following 0.001 µg/ml acrylamide for 6 months
Organism Mus musculus
Characteristics strain: Swiss
gender: Male
age: 11-12 months
agent: acrylamide
dose: 0.001 µg/ml
time: 6 months
tissue: testis
Treatment protocol Acrylamide drinking water solutions were prepared using research-grade acrylamide obtained from Sigma (≥ 99% purity, A9099) diluted in filtered, deionized water at concentrations 0.001, 0.01, 0.1, 1, and 10 ?g/ml. Drinking water containing acrylamide was prepared fresh on a weekly basis, and was substituted for normal drinking water at the commencement of treatment. Test animals were exposed to acrylamide via the drinking water for a period of 1, 6 or 12 months
Growth protocol Adult male Swiss mice were housed under controlled temperature and humidity conditions, 16 h light, 8 h dark, with food and water provided ad libitum. Animals were acclimated for at least 1 month prior to treatment. At each time point, animals were euthanized by CO2 asphyxiation and testes were extracted.
Extracted molecule total RNA
Extraction protocol RNA was extracted from individual testes of acrylamide exposed mice using RNeasy Mini Kit (Qiagen, Chadstone Centre, VIC) according to manufacturers' instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared with Ambion's Illumina RNA Amplification kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Sample name: 23
Data processing The data were normalised using quantile normalisation using BioConductor's lumi package in R statistical software
 
Submission date May 31, 2013
Last update date Jun 01, 2014
Contact name Belinda Nixon
E-mail(s) belinda.nixon@uon.edu.au
Organization name University of Newcastle
Street address University Drive
City Callaghan
State/province NSW
ZIP/Postal code 2308
Country Australia
 
Platform ID GPL6885
Series (1)
GSE47574 Expression data from mouse testis following chronic exposure to acrylamide via the drinking water

Data table header descriptions
ID_REF
VALUE Quantile normalized signal intensity

Data table
ID_REF VALUE
ILMN_1250052 9.619862678
ILMN_3122480 8.627005726
ILMN_2599935 8.281115238
ILMN_2675543 7.726334754
ILMN_2686883 7.760515156
ILMN_2751818 7.721853866
ILMN_2728634 7.844971175
ILMN_3040515 7.741765513
ILMN_2711608 7.724036184
ILMN_1232875 7.706001509
ILMN_1258507 7.880633479
ILMN_2746142 7.678648202
ILMN_1252690 8.08702018
ILMN_2655499 7.720687879
ILMN_1252870 7.788622626
ILMN_1248179 7.790721919
ILMN_2649955 9.182443051
ILMN_2628708 8.596284001
ILMN_3024781 7.736365385
ILMN_2705628 7.73105459

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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