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Sample GSM1152602 Query DataSets for GSM1152602
Status Public on Jun 01, 2014
Title 10µg/ml 6 month rep 1
Sample type RNA
 
Source name mouse testis following 10 µg/ml acrylamide for 6 months
Organism Mus musculus
Characteristics strain: Swiss
gender: Male
age: 11-12 months
agent: acrylamide
dose: 10 µg/ml
time: 6 months
tissue: testis
Treatment protocol Acrylamide drinking water solutions were prepared using research-grade acrylamide obtained from Sigma (≥ 99% purity, A9099) diluted in filtered, deionized water at concentrations 0.001, 0.01, 0.1, 1, and 10 ?g/ml. Drinking water containing acrylamide was prepared fresh on a weekly basis, and was substituted for normal drinking water at the commencement of treatment. Test animals were exposed to acrylamide via the drinking water for a period of 1, 6 or 12 months
Growth protocol Adult male Swiss mice were housed under controlled temperature and humidity conditions, 16 h light, 8 h dark, with food and water provided ad libitum. Animals were acclimated for at least 1 month prior to treatment. At each time point, animals were euthanized by CO2 asphyxiation and testes were extracted.
Extracted molecule total RNA
Extraction protocol RNA was extracted from individual testes of acrylamide exposed mice using RNeasy Mini Kit (Qiagen, Chadstone Centre, VIC) according to manufacturers' instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared with Ambion's Illumina RNA Amplification kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Sample name: 33
Data processing The data were normalised using quantile normalisation using BioConductor's lumi package in R statistical software
 
Submission date May 31, 2013
Last update date Jun 01, 2014
Contact name Belinda Nixon
E-mail(s) belinda.nixon@uon.edu.au
Organization name University of Newcastle
Street address University Drive
City Callaghan
State/province NSW
ZIP/Postal code 2308
Country Australia
 
Platform ID GPL6885
Series (1)
GSE47574 Expression data from mouse testis following chronic exposure to acrylamide via the drinking water

Data table header descriptions
ID_REF
VALUE Quantile normalized signal intensity

Data table
ID_REF VALUE
ILMN_1250052 10.29081028
ILMN_3122480 8.513788221
ILMN_2599935 8.443374356
ILMN_2675543 7.758445141
ILMN_2686883 7.677684317
ILMN_2751818 7.692386745
ILMN_2728634 7.843177782
ILMN_3040515 7.716870798
ILMN_2711608 7.758445141
ILMN_1232875 7.734449422
ILMN_1258507 7.757793544
ILMN_2746142 7.697810916
ILMN_1252690 8.294263421
ILMN_2655499 7.715717892
ILMN_1252870 7.68588575
ILMN_1248179 7.727032231
ILMN_2649955 9.434639513
ILMN_2628708 8.552214236
ILMN_3024781 7.715717892
ILMN_2705628 7.644582229

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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