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Sample GSM1152604 Query DataSets for GSM1152604
Status Public on Jun 01, 2014
Title 10µg/ml 6 month rep 3
Sample type RNA
 
Source name mouse testis following 10 µg/ml acrylamide for 6 months
Organism Mus musculus
Characteristics strain: Swiss
gender: Male
age: 11-12 months
agent: acrylamide
dose: 10 µg/ml
time: 6 months
tissue: testis
Treatment protocol Acrylamide drinking water solutions were prepared using research-grade acrylamide obtained from Sigma (≥ 99% purity, A9099) diluted in filtered, deionized water at concentrations 0.001, 0.01, 0.1, 1, and 10 ?g/ml. Drinking water containing acrylamide was prepared fresh on a weekly basis, and was substituted for normal drinking water at the commencement of treatment. Test animals were exposed to acrylamide via the drinking water for a period of 1, 6 or 12 months
Growth protocol Adult male Swiss mice were housed under controlled temperature and humidity conditions, 16 h light, 8 h dark, with food and water provided ad libitum. Animals were acclimated for at least 1 month prior to treatment. At each time point, animals were euthanized by CO2 asphyxiation and testes were extracted.
Extracted molecule total RNA
Extraction protocol RNA was extracted from individual testes of acrylamide exposed mice using RNeasy Mini Kit (Qiagen, Chadstone Centre, VIC) according to manufacturers' instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared with Ambion's Illumina RNA Amplification kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Sample name: 35
Data processing The data were normalised using quantile normalisation using BioConductor's lumi package in R statistical software
 
Submission date May 31, 2013
Last update date Jun 01, 2014
Contact name Belinda Nixon
E-mail(s) belinda.nixon@uon.edu.au
Organization name University of Newcastle
Street address University Drive
City Callaghan
State/province NSW
ZIP/Postal code 2308
Country Australia
 
Platform ID GPL6885
Series (1)
GSE47574 Expression data from mouse testis following chronic exposure to acrylamide via the drinking water

Data table header descriptions
ID_REF
VALUE Quantile normalized signal intensity

Data table
ID_REF VALUE
ILMN_1250052 10.23721154
ILMN_3122480 8.461049569
ILMN_2599935 8.115375485
ILMN_2675543 7.752065734
ILMN_2686883 7.738029801
ILMN_2751818 7.652352772
ILMN_2728634 7.762367599
ILMN_3040515 7.752819586
ILMN_2711608 7.747673253
ILMN_1232875 7.757889594
ILMN_1258507 7.729982118
ILMN_2746142 7.661372353
ILMN_1252690 8.198586166
ILMN_2655499 7.734849409
ILMN_1252870 7.718326337
ILMN_1248179 7.74698487
ILMN_2649955 9.308477283
ILMN_2628708 8.972907642
ILMN_3024781 7.81015191
ILMN_2705628 7.69654346

Total number of rows: 25697

Table truncated, full table size 624 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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