|
| Status |
Public on Aug 13, 2014 |
| Title |
tg_0309 |
| Sample type |
RNA |
| |
|
| Source name |
tg_E12.5 pooled fore- and hindlimb tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: SHOX transgene
tissue: pooled fore- and hindlimb tissue
age: E12.5
|
| Treatment protocol |
Tissue was stored at -80°C until RNA preparation
|
| Growth protocol |
murine embryonic fore- and hindlimb tissue was dissected at stage E12.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNeasy Kit (Qiagen) according to the manufacturer's instructions, following homogenization using a PT1300 D polytron (Kinematica). DNA was hydrolyzed using the RNAse-free DNAse Kit (Qiagen). Samples from embryonic limbs (E12.5) of the same genotype (wildtype and SHOX transgene) were pooled.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression data from murine SHOX-transgenic pooled fore- and hindlimbs at stage E12.5
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 4, from SAS. Gene expression profiling was performed using arrays of mouse430_2 -type from Affymetrix. A Custom CDF Version 13 with entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization.
|
| |
|
| Submission date |
Jun 13, 2013 |
| Last update date |
Aug 13, 2014 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14661 |
| Series (1) |
| GSE47902 |
Expression profiles of wildtype and SHOX transgenic embryonic mouse limbs |
|
