|
| Status |
Public on Jun 28, 2006 |
| Title |
Germfree Paneth cell 2 |
| Sample type |
RNA |
| |
|
| Source name |
laser capture microdissected Paneth cell from mouse
|
| Organism |
Mus musculus |
| Characteristics |
Strain: NMRI, sex: Male, age: 8 weeks, colonization status: germ-free, Tissue: laser capture microdissected Paneth cell
|
| Biomaterial provider |
Lora Hooper
|
| Treatment protocol |
Mice were maintained under germ-free conditions until sacrifice. Small intestines were snap frozen in OCT and sectioned for laser capture microdissection. Paneth cells were isolated from small intestine by laser capture microdissection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from captured cells (harvested from 3 mice per group) using the Arcturus PicoPure RNA isolation kit, and was quantitated using the RiboGreen RNA quantitation reagent (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
RNAs from three mice were pooled in equal amounts. Duplicate 10 ng RNA aliquots were amplified for each condition using the Arcturus RiboAmp HS kit. Biotinylated cRNAs were generated by substituting the Enzo T7 BioArray Transcript Kit during the last step.
|
| |
|
| Description |
Laser capture microdissected Paneth cell from germ-free NMRI mice
|
| Data processing |
Affymetrix GeneChip software; arrays were scaled to a target intensity of 500
|
| |
|
| Submission date |
Jun 26, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Lora V Hooper |
| E-mail(s) |
Lora.Hooper@UTSouthwestern.edu
|
| Phone |
214-648-7306
|
| Organization name |
The University of Texas Southwestern Medical Center at Dallas
|
| Department |
Immunology
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-9093 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE5156 |
Impact of intestinal colonization on Paneth cell gene expression |
|
| Relations |
| Reanalyzed by |
GSE119085 |
