|
| Status |
Public on Sep 15, 2013 |
| Title |
A/Udorn/72 infected |
| Sample type |
SRA |
| |
|
| Source name |
Alveolar epithelial cell line, A549
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
infection: A/Udorn/72
|
| Treatment protocol |
Cells were either mock infected or infected with either A/Udorn/72 or A/WSN/33 (5 pfu/cell) for 10 hours
|
| Growth protocol |
A549 cells were cultured in F12K media (Gibco) supplemented with 10% cosmic calf serum (Hyclone) and penicillin and streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified and size fractionated using miRNEASY RNA isolation kit and Rneasy MinElute Clean up kit (Qiagen)
Libraries were constructed using 1 microgram of small RNA and the Small RNA Expression Kit (Applied Biosystems)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
AB SOLiD 4 System |
| |
|
| Data processing |
Sequence tags were processed and analyzed using BioScope software package (Applied Biosystems)
MicroRNA sequence tags were normalized by thousand mappable tags
Genome_build: hg19
Supplementary_files_format_and_content: Tab delimited file of microRNA sequence tags per thousand mappable tags
|
| |
|
| Submission date |
Jun 17, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
William Buggele |
| Organization name |
Northwestern University
|
| Street address |
2200 Campus Dr.
|
| City |
Evanston |
| State/province |
IL |
| ZIP/Postal code |
60208 |
| Country |
USA |
| |
|
| Platform ID |
GPL13393 |
| Series (1) |
| GSE48036 |
MicroRNA profiling of influenza A virus infected cells |
|
| Relations |
| BioSample |
SAMN02205229 |
| SRA |
SRX307003 |
