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Sample GSM1167112 Query DataSets for GSM1167112
Status Public on Feb 28, 2014
Title Peripheral blood, normal control [N63]
Sample type RNA
 
Source name Peripheral blood, normal control
Organism Homo sapiens
Characteristics tissue: Peripheral blood
disease status: normal control
Treatment protocol Blood samples were collected in EDTA tubes within 48-hours of acute myocardial infarction or following recruitment into the study. Nucleated cells were fractionated from 5 mL of heparinized blood.
Growth protocol The study samples consisted of whole blood collected from first-time AMI patients within 48-hours post-MI and controls with a normal echocardiogram.
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array (HG-U133_Plus_2). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description normal control group
Data processing The data were analyzed with R/Bioconductor using RMA.
 
Submission date Jun 18, 2013
Last update date Feb 28, 2014
Contact name XING LI
E-mail(s) li.xing@mayo.edu
Organization name Mayo Clinic
Department Health Sciences Research
Street address 200 1st street SW
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
 
Platform ID GPL570
Series (1)
GSE48060 Transcriptome from circulating cells suggests dysregulated pathways associated with long-term recurrent events following first-time myocardial infarction.

Data table header descriptions
ID_REF
VALUE log2 RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 6.130311181
1053_at 6.505399704
117_at 8.577258099
121_at 7.980418568
1255_g_at 2.745030913
1294_at 7.546918138
1316_at 6.636505753
1320_at 3.501974893
1405_i_at 13.18758454
1431_at 3.127574927
1438_at 4.926338225
1487_at 7.592874478
1494_f_at 5.322001884
1552256_a_at 6.186203377
1552257_a_at 6.369250216
1552258_at 6.021625797
1552261_at 3.68175439
1552263_at 8.249632818
1552264_a_at 8.67428519
1552266_at 2.716761506

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM1167112_Nel010163-HG-U133Plus2.cel.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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