|
| Status |
Public on Feb 28, 2014 |
| Title |
Peripheral blood, normal control [N65] |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood, normal control
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Peripheral blood
disease status: normal control
|
| Treatment protocol |
Blood samples were collected in EDTA tubes within 48-hours of acute myocardial infarction or following recruitment into the study. Nucleated cells were fractionated from 5 mL of heparinized blood.
|
| Growth protocol |
The study samples consisted of whole blood collected from first-time AMI patients within 48-hours post-MI and controls with a normal echocardiogram.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Genome U133 Plus 2.0 Array (HG-U133_Plus_2). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
normal control group
|
| Data processing |
The data were analyzed with R/Bioconductor using RMA.
|
| |
|
| Submission date |
Jun 18, 2013 |
| Last update date |
Feb 28, 2014 |
| Contact name |
XING LI |
| E-mail(s) |
li.xing@mayo.edu
|
| Organization name |
Mayo Clinic
|
| Department |
Health Sciences Research
|
| Street address |
200 1st street SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE48060 |
Transcriptome from circulating cells suggests dysregulated pathways associated with long-term recurrent events following first-time myocardial infarction. |
|
