|
Status |
Public on Jul 01, 2013 |
Title |
AKAP95 shRNA, RA-differentiated |
Sample type |
RNA |
|
|
Source name |
E14_AKAP95 KD_differentiated
|
Organism |
Mus musculus |
Characteristics |
cell type: E14 cells infected with: AKAP95 shRNA genotype/variation: AKAP95 knockdown treated with: retinoic acid (RA) differentiation status: RA-differentiated
|
Treatment protocol |
Cells stably infected with viruses expressing indicated shRNAs were either untreated or treated with retinoic acid (RA) for differentiation (four days depletion of LIF followed by four days of 1 µM all-trans RA on monolayer culture.
|
Growth protocol |
E14 cells were cultured on 0.1% gelatinized tissue-culture plates in complete ESC growth medium supplemented with LIF
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were extracted using the RNeasy kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
AKAP95 shRNA, RA-differentiated SAMPLE B; 5110583017_B
|
Data processing |
Results were analyzed by GeneSpring GX 10.0.2. Genes were filtered on Flags Present or Marginal. Signals were normalized against the median in each sample.
|
|
|
Submission date |
Jun 19, 2013 |
Last update date |
Jul 01, 2013 |
Contact name |
Hao Jiang |
E-mail(s) |
haojiang@uab.edu
|
Organization name |
University of Alabama at Birmingham
|
Department |
Biochemistry and Molecular Genetics
|
Street address |
1825 University Blvd
|
City |
Birmingham |
State/province |
AL |
ZIP/Postal code |
35294 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE48128 |
Regulation of transcription by MLL2 complex and MLL complexes-associated protein AKAP95 |
|