|
| Status |
Public on Jul 01, 2013 |
| Title |
AKAP95 shRNA, undifferentiated |
| Sample type |
RNA |
| |
|
| Source name |
E14_AKAP95 KD_undifferentiated
|
| Organism |
Mus musculus |
| Characteristics |
cell type: E14 cells
infected with: AKAP95 shRNA
genotype/variation: AKAP95 knockdown
treated with: none
differentiation status: undifferentiated
|
| Treatment protocol |
Cells stably infected with viruses expressing indicated shRNAs were either untreated or treated with retinoic acid (RA) for differentiation (four days depletion of LIF followed by four days of 1 µM all-trans RA on monolayer culture.
|
| Growth protocol |
E14 cells were cultured on 0.1% gelatinized tissue-culture plates in complete ESC growth medium supplemented with LIF
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using the RNeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
AKAP95 shRNA, undifferentiated
SAMPLE F; 5110583017_F
|
| Data processing |
Results were analyzed by GeneSpring GX 10.0.2. Genes were filtered on Flags Present or Marginal. Signals were normalized against the median in each sample.
|
| |
|
| Submission date |
Jun 19, 2013 |
| Last update date |
Jul 01, 2013 |
| Contact name |
Hao Jiang |
| E-mail(s) |
haojiang@uab.edu
|
| Organization name |
University of Alabama at Birmingham
|
| Department |
Biochemistry and Molecular Genetics
|
| Street address |
1825 University Blvd
|
| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35294 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE48128 |
Regulation of transcription by MLL2 complex and MLL complexes-associated protein AKAP95 |
|
