|
| Status |
Public on Sep 19, 2006 |
| Title |
normal lamb at 20d, biological rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
20 day normal (wild-type) longissimus dorsi total RNA
|
| Organism |
Ovis aries |
| Characteristics |
Genotype: normal wild-type +/+
Age: 20 days of age
|
| Treatment protocol |
Animals were euthanized, longissimus dorsi tissue excised and fixed in RNAlater (Ambion).
|
| Growth protocol |
lambs were allowed to nurse dams until 30 minutes prior to euthanasia
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissue homogenized in 4 M guanidinium thiocyanate, 25 mM EDTA, 1% sodium-N-lauroyl-sarcosine. Homogenates ultracentrifuged on 5.7 M CsCL cushion. RNA purified and DNase treated with Nucleospin RNA II columns (Machery-Nagel Inc).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Bovine Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from callipyge and normal lambs during the onset of muscle hypertrophy phenotype.
|
| Data processing |
Images were interpreted with Microarray Suite version 5.0 (MAS 5.0) in GCOS with no scaling or normalization.
|
| |
|
| Submission date |
Jun 29, 2006 |
| Last update date |
Sep 18, 2006 |
| Contact name |
Christopher A Bidwell |
| E-mail(s) |
cbidwell@purdue.edu
|
| Phone |
765-494-8016
|
| Organization name |
Purdue University
|
| Department |
Animal Sciences
|
| Street address |
125 South Russell Street
|
| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907-2042 |
| Country |
USA |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE5195 |
Analysis of gene expression during the onset of muscle hypertrophy in callipyge lambs: time course |
|
