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Status |
Public on Sep 19, 2006 |
Title |
normal lamb at 20d, biological rep 2 |
Sample type |
RNA |
|
|
Source name |
20 day normal (wild-type) longissimus dorsi total RNA
|
Organism |
Ovis aries |
Characteristics |
Genotype: normal wild-type +/+ Age: 20 days of age
|
Treatment protocol |
Animals were euthanized, longissimus dorsi tissue excised and fixed in RNAlater (Ambion).
|
Growth protocol |
lambs were allowed to nurse dams until 30 minutes prior to euthanasia
|
Extracted molecule |
total RNA |
Extraction protocol |
Tissue homogenized in 4 M guanidinium thiocyanate, 25 mM EDTA, 1% sodium-N-lauroyl-sarcosine. Homogenates ultracentrifuged on 5.7 M CsCL cushion. RNA purified and DNase treated with Nucleospin RNA II columns (Machery-Nagel Inc).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 20 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Bovine Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
Description |
Gene expression data from callipyge and normal lambs during the onset of muscle hypertrophy phenotype.
|
Data processing |
Images were interpreted with Microarray Suite version 5.0 (MAS 5.0) in GCOS with no scaling or normalization.
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Submission date |
Jun 29, 2006 |
Last update date |
Sep 18, 2006 |
Contact name |
Christopher A Bidwell |
E-mail(s) |
cbidwell@purdue.edu
|
Phone |
765-494-8016
|
Organization name |
Purdue University
|
Department |
Animal Sciences
|
Street address |
125 South Russell Street
|
City |
West Lafayette |
State/province |
IN |
ZIP/Postal code |
47907-2042 |
Country |
USA |
|
|
Platform ID |
GPL2112 |
Series (1) |
GSE5195 |
Analysis of gene expression during the onset of muscle hypertrophy in callipyge lambs: time course |
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