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Sample GSM1173624 Query DataSets for GSM1173624
Status Public on Jun 26, 2013
Title knee articular cartilage at T2, biological rep2
Sample type RNA
 
Source name knee articular cartilage in youth
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley
age: youth
time point: T2 (week 4)
tissue: knee articular cartilage
Treatment protocol After grinded under the ice, cartilage tissue were collected for further total RNA extraction.
Growth protocol The cartilage that was harvested individually from the right knee joint were stored at liquid nitrogen temperatures at once with protected by Rnasin inhibitor.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip miRNA 3.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
Description Rattus norvegicus_T2-2
Data processing The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100 (Affymetrix Expression Expression Console v.1.1).
 
Submission date Jun 25, 2013
Last update date Jun 26, 2013
Contact name Xiao Chun Wei
E-mail(s) weixc666@163.com
Phone 86-0351-3365579
Organization name the Second Hospital of Shanxi Medical University
Department Department of Orthopaedics
Lab Shanxi Key Laboratory of Bone and Soft Tissue Injury Repair
Street address No.382, Wuyi Road,Taiyuan City.
City Taiyuan City
State/province Shanxi Shanxi
ZIP/Postal code 030001
Country China
 
Platform ID GPL16384
Series (1)
GSE48266 Expression data from the rat knee articular cartilage at different developmental stages

Data table header descriptions
ID_REF
VALUE RMA signal intensity (rat-only)
Detection

Data table
ID_REF VALUE Detection
hp_rno-let-7a-1_st 0.4854771 A
hp_rno-let-7a-1_x_st 0.8526967 P
hp_rno-let-7a-2_st 0.4520865 A
hp_rno-let-7b_st 1.324862 P
hp_rno-let-7b_x_st 0.2633547 A
hp_rno-let-7c-1_st 0.2426889 A
hp_rno-let-7c-2_st 0.05595016 A
hp_rno-let-7c-2_x_st -0.1287517 A
hp_rno-let-7d_st 0.4508685 P
hp_rno-let-7e_st 0.9083447 P
hp_rno-let-7f-1_st 0.008829243 A
hp_rno-let-7f-1_x_st 0.3689779 P
hp_rno-let-7f-2_st 0.1773286 A
hp_rno-let-7f-2_x_st 0.289107 P
hp_rno-let-7i_st 0.7457196 P
hp_rno-mir-100_st 0.05218023 A
hp_rno-mir-101a_st -0.1593076 A
hp_rno-mir-101a_x_st 0.4477106 A
hp_rno-mir-101b_st -0.1275212 A
hp_rno-mir-103-1_st 0.07083568 A

Total number of rows: 1166

Table truncated, full table size 34 Kbytes.




Supplementary file Size Download File type/resource
GSM1173624_Rattus_norvegicus_T2-2.CEL.gz 803.3 Kb (ftp)(http) CEL
Processed data included within Sample table

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