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Sample GSM1174983 Query DataSets for GSM1174983
Status Public on Feb 07, 2014
Title Rapa Male 4
Sample type RNA
Source name Rapa male liver
Organism Mus musculus
Characteristics strain: C57BL6/N
age: 25 months
gender: male
treatment group: Rapamycin
tissue: liver
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from 25 mg of liver in each sample using Qiagen Rneasy kit following manufacturer’s protocol. RNA quantity was assessed by Nanodrop and RNA quality was assessed by Agilent Bioanalyzer.
Label biotin
Label protocol Biotinylated cRNA were prepared using Ambion kit- Illumina TotalPrep RNA Amplification Kit
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description RM 4; Rapa M754
Data processing Data was processed in matlab by log2 transformation and quantile normalization. Please note that the normalized data was processed with detection p-value <0.05 and also normalized as a large dataset with other arrays that were not included in this Series, resulting a reduced data set of 17,082 data rows.
Submission date Jun 26, 2013
Last update date Feb 07, 2014
Contact name Wilson Chun Yim Fok
Organization name Washington University in St. Louis
Department Hematology
Street address 660 S Euclid Ave
City Saint Louis
State/province MO
ZIP/Postal code 63110
Country USA
Platform ID GPL6885
Series (2)
GSE48331 The effect of short term Rapamycin on the transcriptome of old mouse liver
GSE48334 The effect of Rapamycin on the transcriptome of old mouse liver

Data table header descriptions
VALUE log2 transformation and quantile normalization
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 11.56324395 1
ILMN_2721178 9.790097979 1
ILMN_3033922 10.82857387 1
ILMN_3092673 13.11356047 1
ILMN_2816356 7.374952519 0.94236
ILMN_2808939 11.28925019 1
ILMN_2634564 9.407339472 1
ILMN_2734484 9.980734031 1
ILMN_2952292 8.343929639 1
ILMN_1213681 8.752259371 1
ILMN_2735413 9.837540226 1
ILMN_2735415 10.03608602 1
ILMN_2891688 9.675692456 1
ILMN_2637698 9.548701518 1
ILMN_2674228 8.147141313 1
ILMN_2601546 7.241572084 0.85213
ILMN_1230831 7.217412496 0.83083
ILMN_2848071 10.62306643 1
ILMN_1248518 12.1674499 1
ILMN_2658619 11.40012228 1

Total number of rows: 17082

Table truncated, full table size 497 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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