|
| Status |
Public on Feb 07, 2014 |
| Title |
Rapa Female 10 |
| Sample type |
RNA |
| |
|
| Source name |
Rapa female liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/N
age: 25 months
gender: female
treatment group: Rapamycin
tissue: liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 25 mg of liver in each sample using Qiagen Rneasy kit following manufacturer’s protocol. RNA quantity was assessed by Nanodrop and RNA quality was assessed by Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using Ambion kit- Illumina TotalPrep RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
RF 10; Rapa F1030
|
| Data processing |
Data was processed in matlab by log2 transformation and quantile normalization. Please note that the normalized data was processed with detection p-value <0.05 and also normalized as a large dataset with other arrays that were not included in this Series, resulting a reduced data set of 17,082 data rows.
|
| |
|
| Submission date |
Jun 26, 2013 |
| Last update date |
Feb 07, 2014 |
| Contact name |
Wilson Chun Yim Fok |
| E-mail(s) |
wilson.fok@wustl.edu
|
| Organization name |
Washington University in St. Louis
|
| Department |
Hematology
|
| Street address |
660 S Euclid Ave
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (2) |
| GSE48331 |
The effect of short term Rapamycin on the transcriptome of old mouse liver |
| GSE48334 |
The effect of Rapamycin on the transcriptome of old mouse liver |
|
