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Sample GSM1176479 Query DataSets for GSM1176479
Status Public on Jun 27, 2013
Title Saline + SAM (M+S) 1
Sample type RNA
 
Source name Saline + SAM (M+S) 1, Mouse nucleus accumbens tissue
Organism Mus musculus
Characteristics gender: Male
strain: C57BL/6
age: 5 months
agent: Saline + SAM (M+S)
tissue: nucleus accumbens
Treatment protocol Mice were treated for 7 days intraperitoneally (i.p.) with sterile saline (0.1 mL/10 g body weight) or SAM (4520 mg/kg, 10 mM/kg) 20 minutes prior to cocaine hydrochloride administration (10 mg/kg, i.p.).
Animals (n = 17-22) were randomly assigned into one of the following treatment groups: (1) Saline + Saline “S+S”; (2) SAM + Saline “M+S”; (3) Saline + Cocaine “S+C” and (4) SAM + Cocaine “M+C”.
Locomotor activity was recorded for 90 minutes after the second injection days 1, 3, 5, and 7, and analyzed using VideoMot2 software. Mice were sacrificed 24 h after the end of the repeated treatment.
Extracted molecule total RNA
Extraction protocol RNA was extracted from the mouse NAc using Qiagen RNeasy mini kit in accordance with the prescribed protocol provided with the kit. RNA quantity and quality were assessed using the NanoDrop-1000 spectrophotometer and the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA).
Label biotin
Label protocol Biotinylated cRNA were prepared with the Illumina TotalPrep RNA amplification Kit according to the manufacturer’s instructions using 750 ng of total RNA as a template (Ambion Inc, Austin, TX, USA).
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description SAMPLE 5
2 tissues were combined to a sample
Data processing The raw data was analyzed with Illumina BeadStudio Gene Expression Module v3.3.7. Further data analysis was performed with R version 2.13.0 /Bioconductor software using lumi and limma packages.
 
Submission date Jun 27, 2013
Last update date Jun 27, 2013
Contact name Kaili Anier
E-mail(s) kaili.anier@gmail.com
Organization name University of Tartu
Street address Ravila 19
City Tartu
ZIP/Postal code 50411
Country Estonia
 
Platform ID GPL6885
Series (1)
GSE48365 S-adenosylmethionine modifies cocaine-induced DNA methylation and increases locomotor sensitization in mice

Data table header descriptions
ID_REF
VALUE quantile normalized signal
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 3202.200 0.000
ILMN_2721178 761.600 0.000
ILMN_3033922 1002.200 0.000
ILMN_3092673 3930.600 0.000
ILMN_2816356 32.200 0.023
ILMN_2808939 1085.400 0.000
ILMN_2634564 332.200 0.000
ILMN_2737647 3.500 0.279
ILMN_2734484 724.800 0.000
ILMN_2952292 81.000 0.000
ILMN_2699078 -1.300 0.440
ILMN_1213681 265.200 0.000
ILMN_2735413 -5.000 0.625
ILMN_2735415 -4.200 0.571
ILMN_2891688 1010.400 0.000
ILMN_2637698 1187.500 0.000
ILMN_2674228 312.900 0.000
ILMN_2601546 35.800 0.021
ILMN_1230831 1.500 0.345
ILMN_2848071 17.800 0.080

Total number of rows: 25697

Table truncated, full table size 662 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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