|
| Status |
Public on Jun 27, 2013 |
| Title |
SAM + Cocaine (M+C) 2 |
| Sample type |
RNA |
| |
|
| Source name |
SAM + Cocaine (M+C) 2, Mouse nucleus accumbens tissue
|
| Organism |
Mus musculus |
| Characteristics |
gender: Male
strain: C57BL/6
age: 5 months
agent: SAM + Cocaine (M+C)
tissue: nucleus accumbens
|
| Treatment protocol |
Mice were treated for 7 days intraperitoneally (i.p.) with sterile saline (0.1 mL/10 g body weight) or SAM (4520 mg/kg, 10 mM/kg) 20 minutes prior to cocaine hydrochloride administration (10 mg/kg, i.p.).
Animals (n = 17-22) were randomly assigned into one of the following treatment groups: (1) Saline + Saline “S+S”; (2) SAM + Saline “M+S”; (3) Saline + Cocaine “S+C” and (4) SAM + Cocaine “M+C”.
Locomotor activity was recorded for 90 minutes after the second injection days 1, 3, 5, and 7, and analyzed using VideoMot2 software. Mice were sacrificed 24 h after the end of the repeated treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from the mouse NAc using Qiagen RNeasy mini kit in accordance with the prescribed protocol provided with the kit. RNA quantity and quality were assessed using the NanoDrop-1000 spectrophotometer and the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA amplification Kit according to the manufacturer’s instructions using 750 ng of total RNA as a template (Ambion Inc, Austin, TX, USA).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
SAMPLE 14
2 tissues were combined to a sample
|
| Data processing |
The raw data was analyzed with Illumina BeadStudio Gene Expression Module v3.3.7. Further data analysis was performed with R version 2.13.0 /Bioconductor software using lumi and limma packages.
|
| |
|
| Submission date |
Jun 27, 2013 |
| Last update date |
Jun 27, 2013 |
| Contact name |
Kaili Anier |
| E-mail(s) |
kaili.anier@gmail.com
|
| Organization name |
University of Tartu
|
| Street address |
Ravila 19
|
| City |
Tartu |
| ZIP/Postal code |
50411 |
| Country |
Estonia |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE48365 |
S-adenosylmethionine modifies cocaine-induced DNA methylation and increases locomotor sensitization in mice |
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