|
| Status |
Public on Jul 09, 2014 |
| Title |
alcohol_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA from alcohol-treated embryos
|
| Organism |
Danio rerio |
| Characteristics |
strain: TL
age: 8 hours postfertilization
tissue: whole body
treatment: alcohol
treatment duration: 6 hrs
|
| Treatment protocol |
Embryos were exposed to ethanol by incubation in embryo medium containing 100 mM (0.6% vol./vol.) ethanol from 2 hours post fertilization (hpf) until the completion of the experiment, in Petri dishes wrapped with parafilm and maintained at 28.5ºC.
|
| Growth protocol |
Embryos were grown by incubation in embryo medium, in Petri dishes wrapped with parafilm and maintained at 28.5ºC.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from approximately 20 treated and untreated embryos at 8 hpf using TRIzol reagent (Sigma, St. Louis, MO USA).
|
| Label |
Biotin
|
| Label protocol |
Total RNA was labeled using the 3' IVT express kit from Affymetrix.
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol, except hybridization for 17 hours.
|
| Scan protocol |
Scanned using 3000-7G controlled by Affymetrix GCOS.
|
| Description |
Gene expression data from alcohol-treated embryo.
|
| Data processing |
Affymetrix MAS5 data scaled to intensity of 1000.
|
| |
|
| Submission date |
Jun 27, 2013 |
| Last update date |
Jul 09, 2014 |
| Contact name |
James A Marrs |
| E-mail(s) |
jmarrs@iupui.edu
|
| Organization name |
Indiana University-Purdue University Indianapolis
|
| Department |
Biology
|
| Lab |
SL328
|
| Street address |
723 West Michigan Street
|
| City |
Indianapolis |
| State/province |
IN |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE48380 |
Ethanol exposure disrupts extraembryonic microtubule cytoskeleton and embryonic blastomere cell adhesion, producing epiboly and gastrulation defects |
|
