|
| Status |
Public on Apr 06, 2014 |
| Title |
CLDN18_KnockOut_1 |
| Sample type |
RNA |
| |
|
| Source name |
CLDN18_KnockOut_lung
|
| Organism |
Mus musculus |
| Characteristics |
strain background: mixed 129S6/SvEvTac, C57BL/6J, Balb/c
genotype/variation: CLDN18_KnockOut
age: 3-4 months
gender: Male
tissue: whole lung tissue
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Whole lungs were homogenized in Trizol with a Polytron.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
1μg of RNA from four replicates each from wildtype and claudin-18 knockout whole lung was converted into cRNA and was used for Illumina mouseRef-8 v2 whole genome expression analysis. Expression arrays were carried out at the Southern California Genotyping Consortium (SCGC) at the University of California, Los Angeles.
|
| Label |
Biotin
|
| Label protocol |
IlluminaTotalPrep RNA kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
BeadStudio was used to convert images to raw signal data.
|
| Description |
5388884001_A_Replicate 1
|
| Data processing |
Background correction using variance stabilizing transformation (VST) and quantile normalization were performed using the LUMI package (v.1.14.0) in R v.2.11.1 (2010-05-31).
|
| |
|
| Submission date |
Jul 01, 2013 |
| Last update date |
Jul 17, 2023 |
| Contact name |
Crystal N Marconett |
| E-mail(s) |
cmarcone@usc.edu, cmarconett@coh.org
|
| Phone |
626-218-4357
|
| Organization name |
City of Hope
|
| Department |
Integrative Translational Sciences
|
| Lab |
Crystal Marconett
|
| Street address |
1500 E Duarte Rd
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE48443 |
Knockout mice reveal key roles for claudin-18 in alveolar fluid homeostasis. |
|
