|
| Status |
Public on Jul 03, 2013 |
| Title |
Double knock-out CD71mid Ter119high erythroblasts replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
strain:C57/B6
genotype: Double knock-out
cell type: CD71mid Ter119high erythroblasts
|
| Treatment protocol |
cells are sorted by high speed flow cytometry into Trizol reagent
|
| Growth protocol |
cells are isolated freshly from mice bone marrow
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
SAMPLE 7
|
| Data processing |
The data were normalized with cubic spine using illumina genomestudio.
|
| |
|
| Submission date |
Jul 02, 2013 |
| Last update date |
Jul 03, 2013 |
| Contact name |
Helen He Zhu |
| E-mail(s) |
zhuhecrane@shsmu.edu.cn
|
| Organization name |
School of Medicine, Shanghai Jiaotong University
|
| Department |
Stem Cell Center, Renji Hospital
|
| Lab |
Helen He Zhu Lab
|
| Street address |
3rd Floor, Building 5, 1630 Dongfang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200127 |
| Country |
China |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE48477 |
Microarray of wild type, Shp2 knock-out, Pten knock-out and double knock-out erythroblasts |
|
