|
| Status |
Public on Dec 28, 2013 |
| Title |
H1417-shBRG1 |
| Sample type |
RNA |
| |
|
| Source name |
H1417-shBRG1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: H1417
cell type: Small Cell Lung Cancer (SCLC)
|
| Treatment protocol |
Not special additional treatment
|
| Growth protocol |
Cells where growth on RPMI media with 10% FBS, 1% PS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total mRNA where extrated using Trizol Reagent.
|
| Label |
Cy3
|
| Label protocol |
Amount of nucleic acid labeled: 100ng. Commercial One-Color Microarray-Based Gene Expression Analysis” v.6.6 kit by following manufacturer instructions. Agilent manual G4140-90040 of September 2012. Amplification: by RNA polymerases.
|
| |
|
| Hybridization protocol |
Microarray: SurePrint G3 Human Gene Expression 8x60K v2 Microarray (Agilent microarray design ID 039494)
|
| Scan protocol |
Scanned on an G2505C DNA microarray scanner (Agilent).
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (ver. 10.7)
|
| |
|
| Submission date |
Jul 05, 2013 |
| Last update date |
Dec 29, 2013 |
| Contact name |
Manel Esteller |
| Organization name |
IDIBELL
|
| Department |
PEBC
|
| Lab |
Cancer Epigenetics
|
| Street address |
Hospital Duran i Reynals Av. Gran Via s/n km, 2.7
|
| City |
L'Hospitalet de Llobregat |
| State/province |
Barcelona |
| ZIP/Postal code |
08908 |
| Country |
Spain |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE48559 |
Identification of MAX signature on MAX deficient SCLC cell lines |
|
