|
| Status |
Public on Jul 12, 2013 |
| Title |
CXCR4 knockout 2 |
| Sample type |
RNA |
| |
|
| Source name |
cerebellum
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: cerebellum
genotype/variation: Cxcr4 null
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The cerebellum was dissected for RNA extraction by using TissueLyser and RNeasy Lipid Tissue Kit (Qiagen).
|
| Label |
Cy3
|
| Label protocol |
Messenger RNA molecules were amplified using the MessageAmp II Kit (Ambion) according to the manufacturer's instructions. First strand cDNA was synthesized using 1ug of total RNA and oligo(dT) primers. In vitro transcription was carried out at 37°C for 14 h during which biotinylated UTPs (75 mM Biotin-16-UTP, Ambion) were used for RNA labeling.
|
| |
|
| Hybridization protocol |
After 17-h hybridization at 55°C, beadchips were washed according to recommended protocols from Illumina. FluoroLink Cy3-labeled Streptavidin (Amersham Biosciences) was used to detect expression signals.
|
| Scan protocol |
The BeadStation 500 G system was then used to scan the beadchips.
|
| Description |
This BeadChip targets approximately 25,600 well-annotated RefSeq transcripts, over 19,100 unique genes, and enables the interrogation of eight samples in parallel.
|
| Data processing |
Raw expression values were log2 transformed and median normalized.
|
| |
|
| Submission date |
Jul 11, 2013 |
| Last update date |
Jul 12, 2013 |
| Contact name |
Guo-Jen Huang |
| E-mail(s) |
gjhuang@ntu.edu.tw
|
| Phone |
+886(0)983355800
|
| Organization name |
Chang Gung University
|
| Street address |
259 Wen-Hwa 1st Road
|
| City |
Tao-Yuan |
| ZIP/Postal code |
333 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE48788 |
Ectopic cerebellar cell migration causes maldevelopment of Purkinje cells and abnormal motor behaviour in Cxcr4 null mice |
|
