|
Status |
Public on Jul 11, 2013 |
Title |
Liver-AHCYmut-3 |
Sample type |
RNA |
|
|
Source name |
liver of 4 dpf ahcy mutant larva
|
Organism |
Danio rerio |
Characteristics |
genotype/variation: ahcy -/- agent: vehicle developmental stage: 4 dpf larva tissue: liver
|
Treatment protocol |
At 2 dpf, azacytidine (1 mM) or vehicle was injected into the yolk
|
Growth protocol |
Embryos/larvae were grown at 28 °C in E3 as per standard practice
|
Extracted molecule |
total RNA |
Extraction protocol |
At 4 dpf, larvae were treated with tricaine and livers were removed using thin forceps, and then placed into RNAlater
|
Label |
biotin
|
Label protocol |
RNA was amplified twice and then labeled with Cy3 in accordance with Affymetrix standard protocol
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA was hybridized for 16 hr at 45 °C on GeneChip Zebrafish Genome arrays. GeneChips were washed and stained in the Affymetrix Fludics Station 450
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000, 7G
|
Description |
Gene expression data from liver of 4 dpf ahcy mutant larva M3
|
Data processing |
Probe-level data in CEL files was normalized using GCRMA method implemented in Partek Genomics Suite to get gene-level expression measurements
|
|
|
Submission date |
Jul 11, 2013 |
Last update date |
Jul 11, 2013 |
Contact name |
Randolph Matthews |
E-mail(s) |
matthews@email.chop.edu
|
Phone |
267-426-7223
|
Organization name |
The Children's Hospital of Philadelphia Research Institute
|
Department |
Pediatrics/GI
|
Street address |
3615 Civic Center Blvd
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19147 |
Country |
USA |
|
|
Platform ID |
GPL1319 |
Series (1) |
GSE48804 |
Differentially expressed genes after inhibition of methylation |
|