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Status |
Public on Jan 31, 2008 |
Title |
MRL liver control3 11wks |
Sample type |
RNA |
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|
Source name |
Liver from 11-week-old female MRL +/+ control
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Organism |
Mus musculus |
Characteristics |
Strain: MRL +/+; Gender: female; Age: 11 weeks; Tissue: liver; Experiment: untreated
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the ToTALLY RNA kit from Ambion (Austin, TX) and processed through CHROMA SPIN-100 columns (BD Biosciences Clonetech, Mountain View, CA).
|
Label |
Phycoerythrin
|
Label protocol |
Reverse transcription was performed using 10-25 microg of total RNA and an oligo-dT primer that encodes a T7 RNA polymerase promoter sequence for first-strand cDNA synthesis. Second-strand synthesis converted the cDNA into double-stranded DNA templates for use in an in vitro transcription reaction. Bacteriophage T7 RNA polymerase and biotinylated nucleotides were used to produce biotinylated cRNA.
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Hybridization protocol |
The biotin-labeled antisense cRNA was fragmented to a mean size of 200 bases, and the biotinylated cRNA-fragments served as targets for microarray hybridization. Standard protocols and a GeneChip Hybridization Oven 640 (Affymatrix) were used. Microarrays were washed in a GeneChip Fluidics Station 400 (Affymetrix) using standard and automated protocols. The washing protocol involves exposure to Phycoerythrin-labeled Streptavidin.
|
Scan protocol |
Arrays were scanned on a GeneChip Scanner 3000 (Affymetrix) with GeneChip Operating Software.
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Description |
This sample served as a control in an experiment designed to measure the effects of exposure to dichloroacetyl chloride or dichloroacetic anhydride on liver gene expression in female MRL +/+ mice.
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Data processing |
Data were analyzed using GCOS.
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Submission date |
Jul 05, 2006 |
Last update date |
Jan 03, 2008 |
Contact name |
Rolf Konig |
E-mail(s) |
rokonig@utmb.edu
|
Phone |
(409) 747-0395
|
Fax |
(409) 772-5065
|
Organization name |
UTMB
|
Department |
Microbiology & Immunology
|
Street address |
301 University Blvd.
|
City |
Galveston |
State/province |
TX |
ZIP/Postal code |
77555-1019 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE5241 |
Liver toxicity of dichloroacetyl chloride and dichloroacetic anhydride in female MRL +/+ mice |
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