|
| Status |
Public on Apr 01, 2014 |
| Title |
Liver PRKAR1A ko rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
genotype: PRKAR1A ko
tissue: Liver
genetic background: C57Bl/6J
|
| Treatment protocol |
We disinhibited cAMP-PKA signaling in vivo in mouse liver by ablating hepatic protein kinase A regulatory subunit 1A (prkar1a). Tail vein injection of adenovirus driving CRE recombinase under control of the CMV promoter (AdvCRE) successfully ablated prkar1a and activated PKA-dependent signaling in prkar1afl/fl 14 mice (L-Δprkar1a mice). In contrast, wild-type mice, which were intravenously cannulated and continuously infused for 3 days with D-glucose to achieve fasting glucose levels to match those measured in L-Δprkar1a mice.
|
| Growth protocol |
We disinhibited cAMP-PKA signaling in vivo in mouse liver by ablating hepatic protein kinase A regulatory subunit 1A (prkar1a). Tail vein injection of adenovirus driving CRE recombinase under control of the CMV promoter (AdvCRE) successfully ablated prkar1a and activated PKA-dependent signaling in prkar1afl/fl 14 mice (L-Δprkar1a mice). In contrast, wild-type mice, which were intravenously cannulated and continuously infused for 3 days with D-glucose to achieve fasting glucose levels to match those measured in L-Δprkar1a mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We disinhibited cAMP-PKA signaling in vivo in mouse liver by ablating hepatic protein kinase A regulatory subunit 1A (prkar1a). Tail vein injection of adenovirus driving CRE recombinase under control of the CMV promoter (AdvCRE) successfully ablated prkar1a and activated PKA-dependent signaling in prkar1afl/fl 14 mice (L-Δprkar1a mice). In contrast, wild-type mice, which were intravenously cannulated and continuously infused for 3 days with D-glucose to achieve fasting glucose levels to match those measured in L-Δprkar1a mice.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cRNA was synthesized by the total prep RNA amplification kit from Ambion (Austin, TX). cRNA was quantified and normalized to 150 ng/ul, and then 750 ng was hybridized to each BeadChip.
|
| |
|
| Hybridization protocol |
Biotin-labeled cRNA was synthesized by the total prep RNA amplification kit from Ambion (Austin, TX). cRNA was quantified and normalized to 150 ng/ul, and then 750 ng was hybridized to each BeadChip.
|
| Scan protocol |
The image data was then acquired by scanning the chips on an Illumina iScan.
|
| Description |
rep1
8521433055_B
|
| Data processing |
The raw idat files were uploaded into the Illumina Genomestudio software and the data exported for analysis. Sample and control probe signal intensities were exported from GenomeStudio. These files were imported into R version 2.15.0, quantile normalized, and log2 transformed using the Bioconductor beadarray package, version 2.6.0, and Illumina probe IDs were annotated using the Bioconductor illuminaMousev2.db package, version 1.16.0.
|
| |
|
| Submission date |
Jul 11, 2013 |
| Last update date |
Apr 01, 2014 |
| Contact name |
Stephen Turner |
| Organization name |
Signature Science, LLC
|
| Street address |
1670 Discovery Drive
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22911 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE48815 |
Glucagon regulates Hepatic Kisspeptin1 to Impair Insulin Secretion in Diabetes Mellitus |
|
