|
| Status |
Public on Sep 11, 2014 |
| Title |
Control_6 |
| Sample type |
RNA |
| |
|
| Source name |
Littermate Control
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Spleen
genotype: TRAF3flox/flox (littermate control)
|
| Treatment protocol |
TRAF3flox/flox-CD19+/cre (B-TRAF3-/-) and TRAF3flox/flox (littermate control) mice were generated as described previously (Xie et al., 2007, Immunity 27:253)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from fresh spleen using Trizol reagent
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
control
XP6
|
| Data processing |
Data extracted with Illumina Inc. BeadStudio version 3.2.3 without normalization, then processed in R using the lumi package with lumiExpresso and nuID annotation
|
| |
|
| Submission date |
Jul 11, 2013 |
| Last update date |
Sep 11, 2014 |
| Contact name |
Ronald P. Hart |
| E-mail(s) |
rhart@rutgers.edu
|
| Phone |
848-445-1783
|
| Organization name |
Rutgers University
|
| Department |
Cell Biology & Neuroscience
|
| Street address |
604 Allison Rd Rm B430
|
| City |
Piscataway |
| State/province |
NJ |
| ZIP/Postal code |
08854 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE48818 |
B lymphoma from TRAF3-deficient mice |
|
