A pool of RNA from lung from three female mice with SM22-rtTA transgene on an FVB/N background, raised at Denver altitude (nearly normoxic), fed doxycycline for 8 weeks.
First and second strand complimentary DNA was synthesized using standard techniques. Biotin-labeled antisense complimentary RNA was produced by an in vitro transcription reaction.
Mouse Genome 430 2.0 microarrays (Affymetrix, Foster City, CA) were hybridized with 20 µg cRNA. Target hybridization, washing, staining, and scanning probe arrays were done following an Affymetrix GeneChip Expression Analysis Manual.