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Sample GSM119284 Query DataSets for GSM119284
Status Public on Jul 10, 2006
Title Lung from SM22-dnBMPR2 inducible transgenic females activated 1 week
Sample type RNA
 
Source name Lung
Organism Mus musculus
Characteristics A pool of RNA from lung from three female mice, raised at Denver altitude (nearly normoxic), with SM22-rtTA x tetO7-BMPR2delx4+ transgene activated for 1 week
Extracted molecule total RNA
Extraction protocol Lung tissues were flash-frozen in liquid nitrogen within minutes of death, and RNA extracted using an RNeasy kit (Qiagen)
Label Biotin
Label protocol First and second strand complimentary DNA was synthesized using standard techniques. Biotin-labeled antisense complimentary RNA was produced by an in vitro transcription reaction.
 
Hybridization protocol Mouse Genome 430 2.0 microarrays (Affymetrix, Foster City, CA) were hybridized with 20 µg cRNA. Target hybridization, washing, staining, and scanning probe arrays were done following an Affymetrix GeneChip Expression Analysis Manual.
Description Lung from SM22-dnBMPR2 inducible transgenic females activated 1 week
Data processing dChip, using PM/MM algorithm
 
Submission date Jul 06, 2006
Last update date Jul 07, 2006
Contact name James West
E-mail(s) j.west@vanderbilt.edu
Organization name Vanderbilt University
Department Medicine
Lab Pulmonary
Street address 4200 E 9TH Ave, Box B133
City Nashville
State/province TN
ZIP/Postal code 37201
Country USA
 
Platform ID GPL1261
Series (1)
GSE5255 Loss of BMPR2 Signaling in Smooth Muscle in Mouse Lung

Data table header descriptions
ID_REF
VALUE 1 Week Activation SM22-dnBMPR2 Female

Data table
ID_REF VALUE
1426906_at 212.69
1425451_s_at 586.35
1435330_at 150.61
1450826_a_at 203.08
1456471_x_at 214.12
1454714_x_at 214.83
1419764_at 1004.22
1452231_x_at 417.55
1449824_at 148.55
1422412_x_at 867.31
1421791_at 242.61
1419627_s_at 140.05
1417556_at 78.07
1460227_at 152.73
1444176_at 109.16
1422411_s_at 1434.44
1434798_at 146.74
1434484_at 181.19
1422953_at 166.63
1420249_s_at 463.39

Total number of rows: 45037

Table truncated, full table size 765 Kbytes.




Supplementary data files not provided

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