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Sample GSM119286 Query DataSets for GSM119286
Status Public on Jul 10, 2006
Title Lung from SM22-dnBMPR2 inducible transgenic females activated 8 weeks
Sample type RNA
 
Source name lung
Organism Mus musculus
Characteristics A pool of RNA from lung from three female mice, raised at Denver altitude (nearly normoxic), with SM22-rtTA x tetO7-BMPR2delx4+ transgene activated for 8 weeks
Extracted molecule total RNA
Extraction protocol Lung tissues were flash-frozen in liquid nitrogen within minutes of death, and RNA extracted using an RNeasy kit (Qiagen)
Label Biotin
Label protocol First and second strand complimentary DNA was synthesized using standard techniques. Biotin-labeled antisense complimentary RNA was produced by an in vitro transcription reaction.
 
Hybridization protocol Mouse Genome 430 2.0 microarrays (Affymetrix, Foster City, CA) were hybridized with 20 �g cRNA. Target hybridization, washing, staining, and scanning probe arrays were done following an Affymetrix GeneChip Expression Analysis Manual.
Description Lung from SM22-dnBMPR2 inducible transgenic females activated 8 weeks
Data processing dChip, using PM/MM algorithm
 
Submission date Jul 06, 2006
Last update date Jul 07, 2006
Contact name James West
E-mail(s) j.west@vanderbilt.edu
Organization name Vanderbilt University
Department Medicine
Lab Pulmonary
Street address 4200 E 9TH Ave, Box B133
City Nashville
State/province TN
ZIP/Postal code 37201
Country USA
 
Platform ID GPL1261
Series (1)
GSE5255 Loss of BMPR2 Signaling in Smooth Muscle in Mouse Lung

Data table header descriptions
ID_REF
VALUE Lung from SM22-dnBMPR2 inducible transgenic females activated 8 weeks

Data table
ID_REF VALUE
1426906_at 267.2
1425451_s_at 3335.59
1435330_at 234.18
1450826_a_at 1394.26
1456471_x_at 202.83
1454714_x_at 212.92
1419764_at 3759.19
1452231_x_at 501.03
1449824_at 109.01
1422412_x_at 3746.83
1421791_at 853.14
1419627_s_at 660.52
1417556_at 429.89
1460227_at 671.97
1444176_at 418.47
1422411_s_at 4548.92
1434798_at 518.75
1434484_at 504.38
1422953_at 610.76
1420249_s_at 1564.1

Total number of rows: 45037

Table truncated, full table size 765 Kbytes.




Supplementary data files not provided

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