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Sample GSM119287 Query DataSets for GSM119287
Status Public on Jul 10, 2006
Title Lung from SM22-dnBMPR2 inducible transgenic males activated 8 weeks
Sample type RNA
 
Source name lung
Organism Mus musculus
Characteristics A pool of RNA from lung from three male mice, raised at Denver altitude (nearly normoxic), with SM22-rtTA x tetO7-BMPR2delx4+ transgene activated for 8 weeks; mice are 12 weeks old when sac'd.
Extracted molecule total RNA
Extraction protocol Lung tissues were flash-frozen in liquid nitrogen within minutes of death, and RNA extracted using an RNeasy kit (Qiagen)
Label Biotin
Label protocol First and second strand complimentary DNA was synthesized using standard techniques. Biotin-labeled antisense complimentary RNA was produced by an in vitro transcription reaction.
 
Hybridization protocol Mouse Genome 430 2.0 microarrays (Affymetrix, Foster City, CA) were hybridized with 20 �g cRNA. Target hybridization, washing, staining, and scanning probe arrays were done following an Affymetrix GeneChip Expression Analysis Manual.
Description Lung from SM22-dnBMPR2 inducible transgenic males activated 8 weeks
Data processing dChip, using PM/MM algorithm
 
Submission date Jul 06, 2006
Last update date Jul 07, 2006
Contact name James West
E-mail(s) j.west@vanderbilt.edu
Organization name Vanderbilt University
Department Medicine
Lab Pulmonary
Street address 4200 E 9TH Ave, Box B133
City Nashville
State/province TN
ZIP/Postal code 37201
Country USA
 
Platform ID GPL1261
Series (1)
GSE5255 Loss of BMPR2 Signaling in Smooth Muscle in Mouse Lung

Data table header descriptions
ID_REF
VALUE 8 week SM22-dnBMPR2 males

Data table
ID_REF VALUE
1426906_at 585.26
1425451_s_at 2189.91
1435330_at 265.43
1450826_a_at 1204.18
1456471_x_at 258.09
1454714_x_at 267.38
1419764_at 2762.51
1452231_x_at 773.99
1449824_at 491.54
1422412_x_at 2942.25
1421791_at 488.7
1419627_s_at 516.18
1417556_at 343.76
1460227_at 452.12
1444176_at 363.44
1422411_s_at 3690.76
1434798_at 336.44
1434484_at 667.44
1422953_at 569.54
1420249_s_at 1036.33

Total number of rows: 45037

Table truncated, full table size 763 Kbytes.




Supplementary data files not provided

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