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Sample GSM1193303 Query DataSets for GSM1193303
Status Public on Jun 30, 2014
Title Normal Volunteer, Replicate 3
Sample type RNA
 
Source name Alveolar macrophage isolated from Normal Volunteer
Organism Homo sapiens
Characteristics diagnosis: Normal
tissue: Alveolar Macrophage
Extracted molecule total RNA
Extraction protocol Total RNA extraction.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Affymetrix Expression Analysis Technical Manual, 2005, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray scanner.
Data processing The data were analyzed with Affymetrix apt version 1.12, GeneSpring 11.5.1 . Apt was used to determine present/absent calls and GeneSpring RMA normalization was applied.
 
Submission date Jul 22, 2013
Last update date Sep 01, 2016
Contact name Eric Billings
Organization name NHLBI
Street address 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20874
Country USA
 
Platform ID GPL96
Series (1)
GSE49072 Alveolar Macrophage Gene Expression in Human Pulmonary Fibrosis
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE RMA normalized expression

Data table
ID_REF VALUE
AFFX-BioB-5_at 0.051611423
AFFX-BioB-M_at 0.011517525
AFFX-BioB-3_at 0.12086153
AFFX-BioC-5_at -0.08819008
AFFX-BioC-3_at 0.068992615
AFFX-BioDn-5_at -0.19771767
AFFX-BioDn-3_at -0.098189354
AFFX-CreX-5_at -0.17305851
AFFX-CreX-3_at -0.14925194
AFFX-DapX-5_at -0.054678917
AFFX-DapX-M_at -0.08026075
AFFX-DapX-3_at -0.001805306
AFFX-LysX-5_at 0.061738968
AFFX-LysX-M_at 0.009153843
AFFX-LysX-3_at -0.014266491
AFFX-PheX-5_at 0.018170357
AFFX-PheX-M_at 0.08852148
AFFX-PheX-3_at 0.16599035
AFFX-ThrX-5_at 0.07520485
AFFX-ThrX-M_at 0.015249252

Total number of rows: 22283

Table truncated, full table size 488 Kbytes.




Supplementary file Size Download File type/resource
GSM1193303_RIPF-14.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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