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Sample GSM1193329 Query DataSets for GSM1193329
Status Public on Jun 30, 2014
Title Normal Volunteer, Replicate 19
Sample type RNA
 
Source name Alveolar macrophage isolated from Normal Volunteer
Organism Homo sapiens
Characteristics diagnosis: Normal
tissue: Alveolar Macrophage
Extracted molecule total RNA
Extraction protocol Total RNA extraction.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Affymetrix Expression Analysis Technical Manual, 2005, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray scanner.
Data processing The data were analyzed with Affymetrix apt version 1.12, GeneSpring 11.5.1 . Apt was used to determine present/absent calls and GeneSpring RMA normalization was applied.
 
Submission date Jul 22, 2013
Last update date Sep 01, 2016
Contact name Eric Billings
Organization name NHLBI
Street address 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20874
Country USA
 
Platform ID GPL96
Series (1)
GSE49072 Alveolar Macrophage Gene Expression in Human Pulmonary Fibrosis
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE RMA normalized expression

Data table
ID_REF VALUE
AFFX-BioB-5_at 0.006124973
AFFX-BioB-M_at -0.027873993
AFFX-BioB-3_at 0.13889933
AFFX-BioC-5_at -0.036221504
AFFX-BioC-3_at -0.14073706
AFFX-BioDn-5_at -0.23450089
AFFX-BioDn-3_at -0.04212475
AFFX-CreX-5_at -0.2158308
AFFX-CreX-3_at 0.04133892
AFFX-DapX-5_at -0.034406185
AFFX-DapX-M_at -0.03747654
AFFX-DapX-3_at 0.03350973
AFFX-LysX-5_at -0.05449629
AFFX-LysX-M_at -0.044566154
AFFX-LysX-3_at -0.09130764
AFFX-PheX-5_at -0.004515648
AFFX-PheX-M_at -0.04077339
AFFX-PheX-3_at 0.14221573
AFFX-ThrX-5_at 0.05961895
AFFX-ThrX-M_at -0.040941715

Total number of rows: 22283

Table truncated, full table size 492 Kbytes.




Supplementary file Size Download File type/resource
GSM1193329_RIPF-39.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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