NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1193372 Query DataSets for GSM1193372
Status Public on Jun 30, 2014
Title Spontaneous IPF, Replicate 11
Sample type RNA
 
Source name Alveolar macrophage isolated from Spontaneous IPF
Organism Homo sapiens
Characteristics diagnosis: Spontaneous Idiopathic Pulmonary Fibrosis (IPF)
tissue: Alveolar Macrophage
Extracted molecule total RNA
Extraction protocol Total RNA extraction.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 ug total RNA (Affymetrix Expression Analysis Technical Manual, 2005, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray scanner.
Data processing The data were analyzed with Affymetrix apt version 1.12, GeneSpring 11.5.1 . Apt was used to determine present/absent calls and GeneSpring RMA normalization was applied.
 
Submission date Jul 22, 2013
Last update date Sep 01, 2016
Contact name Eric Billings
Organization name NHLBI
Street address 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20874
Country USA
 
Platform ID GPL96
Series (1)
GSE49072 Alveolar Macrophage Gene Expression in Human Pulmonary Fibrosis
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF
VALUE RMA normalized expression

Data table
ID_REF VALUE
AFFX-BioB-5_at 0.13230228
AFFX-BioB-M_at 0.14971352
AFFX-BioB-3_at 0.17061472
AFFX-BioC-5_at 0.24306107
AFFX-BioC-3_at 0.26067734
AFFX-BioDn-5_at 0.6124706
AFFX-BioDn-3_at 0.1672039
AFFX-CreX-5_at 0.28915596
AFFX-CreX-3_at 0.20865726
AFFX-DapX-5_at -0.055771828
AFFX-DapX-M_at -0.0904212
AFFX-DapX-3_at -0.11639881
AFFX-LysX-5_at -0.080997944
AFFX-LysX-M_at -0.15137291
AFFX-LysX-3_at -0.15983534
AFFX-PheX-5_at -0.12756395
AFFX-PheX-M_at -0.006121635
AFFX-PheX-3_at -0.3041525
AFFX-ThrX-5_at -0.24066162
AFFX-ThrX-M_at -0.13860273

Total number of rows: 22283

Table truncated, full table size 488 Kbytes.




Supplementary file Size Download File type/resource
GSM1193372_RIPF-81A.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap